Justin Timberlake & Jessica Biel on the brink of a break-up?

Justin Timberlake‘s biggest fan and wife, Jessica Biel, wasn’t present for his appearance on Saturday Night Live over the weekend leading partygoer’s at the after-party to question whether the couple has broken up, RadarOnline.com is exclusively reporting.

Reports swirled that there’s trouble in paradise surrounding the A-list couple when Timberlake was recently spotted without his wedding band on during his new tour and Biel’s absence on Saturday night has only fueled the fire.

“Justin was solo again at the Saturday Night Live after party and he stayed until nearly 5 a.m.,” a fellow partygoer exclusively told Radar.

“Everyone was buzzing about her absence.”

Biel was also noticeably absent from Timberlake’s American Music Awards appearance in Nov. which she addressed on Twitter saying, “I’m watching from home tonight. Calm down, Internet.”

The partygoer also reveals that Biel didn’t accompany her husband to the show’s rehearsal’s on Friday.

“She wasn’t with him at rehearsals on Friday either, which was sort of weird,” the source said.

“And it’s not that Justin was flirting with other women or anything at the party but something just didn’t seem right. It was literally what everyone was talking about; whether he and Jessica have split.”

Timberlake did have his wedding band on at the party but barely spoke of his wife, the insider revealed. The power couple wed in October, 2012.

Do you think there’s a breakup on the brink for Timberlake and Biel?